ANALISIS RESIDU ANTIBIOTIK KLORAMFENIKOL DALAM DAGING IKAN GURAMI (Osphronemus gouramy, Lac) MENGGUNAKAN METODE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

The use of antibiotic in food animals is not only for terapeutic purposes, but also for non therapeutic purposes. the use of antibiotic for no-therapeutic purposes is done by adding antibiotic to feed (food additives) that is aimed as growth promotor to improve livestock production. antibiotic which is often used in animals as a growth factor is chloramfenicol (CAP). The use of antibiotic in farm animals for non-therapeutic purpose has potancy to generate accumulation of antibiotic residues in tissues and organs of these animals. humans who consume animals products that contain antiniotic residuesmay cause adverse effects to health, because it can cause allergic reaction irreversible type bone narrow depression that can kead aplastic anemia, and even CAP resistance in humans. Fishery product whiach frequently uses antibiotic to increase its production is residues oin gouramiusisng HPLC (High Performance Liuqid Chromatography) ethod, then its concentration will be compared with MRL (Maximum residues Limits) antibiotic in foodstuffs of animal origin listed in iNdonesia National Standards (SNI) No. 01-6366-2000. The calibration curve showed a good linearity in the concentration range from 5 to 40 ng/ml with r = 0.9995. we found CAP residues in gourami fish sold in three traditional market were 0.276; 0.261; 1.168 ng/g respectively. The shows that CAP residues level not exceed the MRL set by SNI (0.01 mg/kg), but it was found one of three levels of CAP did not met the requirements of the European Union (0.3 mikrog/kg).