To establish the regeneration system of sweet sorghum immature embryos

Abstract

Sweet sorghum is an important forage and energy crop. In this experiment, sweet sorghum immature embryos were taken to be explants, through inducting and regenerating for sweet sorghum callus of different genotypes, to obtain the best culture medium formula and create appropriate conditions for establishing efficient regeneration system. The result showed that, for callus induction, the response of immature embryos varied with the genotype, but the medium is not. Of these, Xinliang 52 showed the highest frequency of callus induction under appropriate culture conditions. As regards regenerable callus formation, the response of callus varied with the genotype and medium. 07-27 regeneration occurred at high frequencies when MSR and MBR were added in the regeneration medium. And there is nothing on NBI medium for the whole genotypes. So NBI is not suitable for callus regeneration culture.