Abstract
Super-resolution microscopy enables the high-precision localization of proteins. Therefore, it is possible to investigate the spatial organization of proteins within the nucleus to understand how their organization relates to regulation and function. Here, we present methodology for single-molecule localization microscopy and cluster analysis where we cover sample preparation, image acquisition, and data analysis.
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dos Santos, Á., Gough, R. E., Wang, L., & Toseland, C. P. (2022). Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis. In Methods in Molecular Biology (Vol. 2476, pp. 293–309). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2221-6_20
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