Abstract
In this chapter we will describe the use of the tubular network assay as a useful tool in the evaluation of cancer neoangiogenesis (as reported by Arnaoutova et al. [Angiogenesis 12:267–274, 2009]). Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. A detailed working protocol applicable to the study of any type of endothelial cells is provided below and in addition, details on data interpretation analysis and troubleshooting.
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Casamassimi, A., De Nigris, F., Schiano, C., & Napoli, C. (2015). Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis. In Handbook of Vascular Biology Techniques (pp. 13–22). Springer Netherlands. https://doi.org/10.1007/978-94-017-9716-0_2
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