Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-Based Approach

Abstract

4-Hydroxenonenal (HNE) is one of the major α,β-unsaturated aldehyde products of lipid peroxidation. HNE can form conjugates with macromolecules, including protein, and thereby alter their function. HNE and its conjugation with proteins are increased in aging and age-related diseases. To elucidate how HNE is involved in these aging-related pathophysiological changes, it is necessary to assess HNE modification of proteins. Here a simple and convenient Western-blot based method is presented to detect HNE modification of proteins in tissues of aging mice.