Protein-storage vacuoles (PSVs) are specialized vacuoles that sequester large amounts of storage proteins. During seed development, PSVs are formed de novo and/or from preexisting lytic vacuoles. Seed PSVs can be subdivided into four distinct compartments: membrane, globoid, matrix, and crystalloid. In this chapter, we introduce easy methods for isolation of PSVs and their membranes from pumpkin seeds. These methods facilitate the identification and characterization of PSV components.
CITATION STYLE
Shimada, T., & Hara-Nishimura, I. (2017). Isolation of protein storage vacuoles and their membranes. In Methods in Molecular Biology (Vol. 1511, pp. 163–168). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6533-5_13
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