Escherichia coli K1 Invasion Increases Human Brain Microvascular Endothelial Cell Monolayer Permeability by Disassembling Vascular-Endothelial Cadherins at Tight Junctions

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Abstract

We investigated the permeability changes that occur in the human brain microvascular endothelial cell (HBMEC) monolayer, an in vitro model of the blood-brain barrier, during Escherichia coli K1 infection. An increase in permeability of HBMECs and a decrease in transendothelial electrical resistance were observed. These permeability changes occurred only when HBMECs were infected with E. coli expressing outer membrane protein A (OmpA) and preceded the traversal of bacteria across the monolayer. Activated protein kinase C (PKC)-α interacts with vascular-endothelial cadherins (VECs) at the tight junctions of HBMECs, resulting in the dissociation of β-catenins from VECs and leading to the increased permeability of the HBMEC monolayer. Overexpression of a dominant negative form of PKC-α in HBMECs blocked the E. coli-induced increase in permeability of HBMECs. Anti-OmpA and anti-OmpA receptor antibodies exerted inhibition of E. coli-induced permeability of HBMEC monolayers. This inhibition was the result of the absence of PKC-α activation in HBMECs treated with the antibodies.

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Sukumaran, S. K., & Prasadarao, N. V. (2003). Escherichia coli K1 Invasion Increases Human Brain Microvascular Endothelial Cell Monolayer Permeability by Disassembling Vascular-Endothelial Cadherins at Tight Junctions. Journal of Infectious Diseases, 188(9), 1295–1309. https://doi.org/10.1086/379042

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