Fluorine-19 NMR studies of glucosyl fluoride transport in human erythrocytes

14Citations
Citations of this article
13Readers
Mendeley users who have this article in their library.

Abstract

Fluorine-19 magnetization transfer studies have been used to measure the transport rate of glucopyranosyl fluorides under equilibrium exchange conditions. Although rate constants and permeabilities could be determined for beta-D-glucopyranosyl fluoride, the exchange rate for alpha-D-glucopyranosyl fluoride was found to be too slow for determination using this method. The time-dependent decomposition of the beta-glucopyranosyl fluoride also limits the accuracy of the numerical results for this species; however, it is clear that the permeabilities of the alpha and beta forms differ significantly, i.e., P beta > P alpha. This observation is in contrast to recent observations for n-fluoro-n-deoxyglucose, for which P alpha > P beta for n = 2, 3, 4, or 6. The difference can be explained in terms of a simple alternating conformation model in which one of the conformations (with an external sugar-binding site) exhibits a preference for the beta form of the molecule, while the second conformation (with an internal sugar binding site) exhibits a preference for the alpha form. Fluorine/hydroxyl substitutions unmask these preferences by selectively reducing the binding to one of the conformations, depending on the specific site of fluorination. © 1995, The Biophysical Society. All rights reserved.

Cite

CITATION STYLE

APA

London, R. E., & Gabel, S. A. (1995). Fluorine-19 NMR studies of glucosyl fluoride transport in human erythrocytes. Biophysical Journal, 69(5), 1814–1818. https://doi.org/10.1016/S0006-3495(95)80051-2

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free