Transposable elements (TEs) are major components of plant genome and are reported to play significant roles in functional genome diversity and phenotypic variations. Several TEs are highly polymorphic for insert location in the genome and this facilitates development of TE-based markers for various genotyping purposes. Considering this, a genome-wide analysis was performed in the model plant foxtail millet. A total of 30,706 TEs were identified and classified as DNA transposons (24,386), full-length Copia type (1,038), partial or solo Copia type (10,118), full-length Gypsy type (1,570), partial or solo Gypsy type (23,293) and Long- and Short-Interspersed Nuclear Elements (3,659 and 53, respectively). Further, 20,278 TE-based markers were developed, namely Retrotransposon-Based Insertion Polymorphisms (4,801, approximately 24%), Inter-Retrotransposon Amplified Polymorphisms (3,239, approximately 16%), Repeat Junction Markers (4,451, approximately 22%), Repeat Junction-Junction Markers (329, approximately 2%), Insertion-Site-Based Polymorphisms (7,401, approximately 36%) and Retrotransposon-Microsatellite Amplified Polymorphisms (57, 0.2%). A total of 134 Repeat Junction Markers were screened in 96 accessions of Setaria italica and 3 wild Setaria accessions of which 30 showed polymorphism. Moreover, an open access database for these developed resources was constructed (Foxtail millet Transposable Elements-based Marker Database; http://22.214.171.124/ltrdb/index.html). Taken together, this study would serve as a valuable resource for large-scale genotyping applications in foxtail millet and related grass species.
Yadav, C. B., Bonthala, V. S., Muthamilarasan, M., Pandey, G., Khan, Y., & Prasad, M. (2015). Genome-wide development of transposable elements-based markers in foxtail millet and construction of an integrated database. DNA Research, 22(1), 79–90. https://doi.org/10.1093/dnares/dsu039