Single functional human CuZnSOD gene encodes two species of mRNA differing in size by 200 nucleotides in the 3′-untranslated region (UTR). We studied the expression of the CuZnSOD cDNA with different 3′- and 5′-UTR. Deletion in the 5′-end does not affect the expression of the enzyme, however, deletion in the 3′-UTR decreases the level of expression of CuZnSOD. The plasmids containing the long CuZnSOD cDNA with all polyadenylation signal sequences utilize primarily the last polyadenylation site and give a long mRNA, which produces three times more enzyme than the short mRNA lacking the last polyadenylation site and the AU-rich region. © 1995.
Kilk, A., Laan, M., & Torp, A. (1995). Human CuZn superoxide dismutase enzymatic activity in cells is regulated by the length of the mRNA. FEBS Letters, 362(3), 323–327. https://doi.org/10.1016/0014-5793(95)00266-C