Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium

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Abstract

© 2015 The Author(s).This article is published with open access at Springerlink.com. The effect of selenium applied as sodium selenite (Na 2 SeO 3 ) on the cytotoxicity of vanadyl sulphate (VOSO 4 ) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na 2 SeO 3 at low doses (0.5 and 1 μM) can enhance 100 μM VOSO 4 -induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na 2 SeO 3 with VOSO 4 and 1 μM Na 2 SeO 3 with VOSO 4 . Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO 4 alone. In turn, a majority of cells co-treated with VOSO 4 and 1 μM Na 2 SeO 3 were elongated, and exhibited cytoplasmic vacuolization. These results warn of the potential contribution of inorganic selenium to vanadium-induced toxicity.

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Zwolak, I. (2015). Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium. Bulletin of Environmental Contamination and Toxicology, 95(5), 593–598. https://doi.org/10.1007/s00128-015-1615-4

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