Regulatory T cells expressing the transcription factor Foxp3 require acquisition of a specific hypomethylation pattern to ensure optimal functional commitment, limited lineage plasticity, and long-term maintenance of tolerance. A better understanding of the molecular mechanisms involved in the generation of these epigenetic changes in vivo will contribute to the clinical exploitation of Foxp3(+) Treg. Here, we show that both in vitro and in vivo generated antigen-specific Foxp3(+) Treg can acquire Treg-specific epigenetic characteristics and prevent skin graft rejection in an animal model.
Hilbrands, R., Chen, Y., Kendal, A. R., Adams, E., Cobbold, S. P., Waldmann, H., & Howie, D. (2016). Induced Foxp3+ T cells colonizing tolerated allografts exhibit the hypomethylation pattern typical of mature regulatory T cells. Frontiers in Immunology, 7(APR). https://doi.org/10.3389/fimmu.2016.00124