Potassium conduction through unblocked inwardly rectifying (IRK1, Kir2.1) potassium channels was measured in inside-out patches from Xenopus oocytes, after removal of polyamine-induced strong inward rectification. Unblocked IRK1 channel current-voltage (I-V) relations show very mild inward rectification in symmetrical solutions, are linearized in nonsymmetrical solutions that bring the K+ reversal potential to extreme negative values, and follow Goldman-Hodgkin-Katz constant field equation at extreme positive E(K). When intracellular K+ concentration (K(IN)) was varied, at constant extracellular K+ concentration (K(OUT)) the conductance at the reversal potential (G(REV)) followed closely the predictions of the Goldman-Hodgkin- Katz constant field equation at low concentrations and saturated sharply at concentrations of >150 mM. Similarly, when K(OUT) was varied, at constant K(IN), G(REV) saturated at concentrations of >150 mM. A square-root dependence of conductance on K(OUT) is a well-known property of inward rectifier potassium channels and is a property of the open channel. A nonsymmetrical two-site three-barrier model can qualitatively explain both the I-V relations and the [K+] dependence of conductance of open IRK1 (Kir2.1) channels.
Lopatin, A. N., & Nichols, C. G. (1996). [K+] dependence of open-channel conductance in cloned inward rectifier potassium channels (IRK1, Kir2.1). Biophysical Journal, 71(2), 682–694. https://doi.org/10.1016/S0006-3495(96)79268-8