Rates of uptake of serine and of adenine were measured as a function of cell size, and therefore age, in asynchronous, exponential phase cultures of diploid Saccharomyces cerevisiae strain Y55. In both cases, uptake rates were constant during the initial third of the cell cycle and doubled during the S period in the middle part of the cycle to a constant value during the final third. Cell size and age at mid-step doubling were indistinguishable for serine and adenine uptake, and occurred during the period of DNA synthesis. The results extend an earlier hypothesis of constancy of cell growth rates (mass accumulation rates) and rates of uptake of all or almost all compounds into cells in exponential phase growth to one of piecewise constancy, with an abrupt doubling of growth and uptake rates during DNA synthesis. © 1977, The Biophysical Society. All rights reserved.
Kubitschek, H. E., & Edvenson, R. W. (1977). Midcycle doubling of uptake rates of adenine and serine in Saccharomyces cerevisiae. Biophysical Journal, 20(1), 15–22. https://doi.org/10.1016/S0006-3495(77)85533-1