Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli

  • Messerschmidt S
  • Schindler D
  • Zumkeller C
  • et al.
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Abstract

� 2016 Messerschmidt, Schindler, Zumkeller, Kemter, Schallopp and Waldminghaus. Learning by building is one of the core ideas of synthetic biology research. Consequently, building synthetic chromosomes is the way to fully understand chromosome characteristics. The last years have seen exciting synthetic chromosome studies. We had previously introduced the synthetic secondary chromosome synVicII in Escherichia coli. It is based on the replication mechanism of the secondary chromosome in Vibrio cholerae. Here, we present a detailed analysis of its genetic characteristics and a selection approach to optimize replicon stability. We p robe the origin diversity of secondary chromosomes from Vibrionaceae by construction of several new respective replicons. Finally, we present a synVicII version 2.0 with several innovations including its full compatibility with the popular modular cloning (MoClo) assembly system.

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Messerschmidt, S. J., Schindler, D., Zumkeller, C. M., Kemter, F. S., Schallopp, N., & Waldminghaus, T. (2016). Optimization and Characterization of the Synthetic Secondary Chromosome synVicII in Escherichia coli. Frontiers in Bioengineering and Biotechnology, 4. https://doi.org/10.3389/fbioe.2016.00096

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