Pharmacology of Ca2+ release from red beet microsomes suggests the presence of ryanodine receptor homologs in higher plants

Abstract

Cyclic ADP-ribose (cADPR) is known to release Ca2+ from plant vacuoles, implying that this NAD+ metabolite may possess a second messenger role in plants. The degree to which the plant cADPR-gated Ca2+ release mechanism resembles cADPR action in animals has been evaluated. cADPR-elicited Ca2+ release from red beet microsomes was inhibited by 1 mM procaine but insensitive to heparin. Furthermore, pre-release of Ca2+ from red beet vesicles by either 5 mM caffeine or micromolar levels of ryanodine precluded further Ca2+ mobilisation by cADPR. Thus, this study argues strongly for conservation between the plant and animal cADPR-elicited Ca2+ release mechanism.