Processing of transcripts of a dimeric tRNA gene in yeast uses the nuclease responsible for maturation of the 3′ termini upon 5 S and 37 S precursor rRNAs

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Abstract

The rna82 mutation of Saccharomyces cerevisiae inactivates an RNA processing activity responsible for maturation of 3′-terminal sequences upon 5 S and 37 S ribosomal RNA precursors. This study describes a difference in the processing of transcripts of an S. cerevisiae dimeric tRNA gene (tRNAArg-tRNAAsp) in RNA polymerase III in vitro transcription extracts prepared from rna82 and wild-type cells. The mutant extract accumulated additional processing intermediates containing tRNAArg sequences as compared to the extract from wild-type cells. The structure of these intermediates revealed a defect in removal of the 10 nucleotides left 3′ to the tRNAArg sequence by the RNase P cleavage immediately 5′ to tRNAAsp. This is the first demonstration of a mutational defect affecting maturation of 3′ sequences upon a eukaryotic tRNA precursor. © 1989.

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Piper, P. W., & Stråby, K. B. (1989). Processing of transcripts of a dimeric tRNA gene in yeast uses the nuclease responsible for maturation of the 3′ termini upon 5 S and 37 S precursor rRNAs. FEBS Letters, 250(2), 311–316. https://doi.org/10.1016/0014-5793(89)80745-8

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