Protective effects of kahweol and cafestol against hydrogen peroxide-induced oxidative stress and DNA damage

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Abstract

There is an increasing evidence that oxidative stress is implicated in the processes of inflammation and carcinogenesis. It has been shown that kahweol and cafestol, coffee-specific diterpenes, exhibit chemoprotective effects. This study investigated the effects of kahweol and cafestol, coffee-specific diterpenes, on the hydrogen peroxide (H 2 O 2 )-induced oxidative stress and DNA damage in NIH3T3 cells. When the cells were treated with kahweol or cafestol, cytotoxicity, lipid peroxidation, and reactive oxygen species production induced by H 2 O 2 were markedly reduced in a dose-dependent manner. Moreover, kahweol and cafestol were shown to be highly protected against H 2 O 2 -induced oxidative DNA damage as determined by the Comet (single cell gel electrophoresis) assay and the measurement of 8-oxoguanine content in NIH3T3 cells. Kahweol and cafestol also protected hydroxyl radical-induced 2-deoxy-d-ribose degradation by ferric ion-nitrilotriacetic acid and H 2 O 2 . In addition, kahweol and cafestol efficiently removed the superoxide anion generated from the xanthine/xanthine oxidase system. These results suggest that kahweol and cafestol are effective in protecting against H 2 O 2 -induced oxidative stress and DNA damage, probably via scavenging free oxygen radicals, and that kahweol and cafestol act as antioxidants. © 2007 Elsevier Ireland Ltd. All rights reserved.

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APA

Lee, K. J., & Jeong, H. G. (2007). Protective effects of kahweol and cafestol against hydrogen peroxide-induced oxidative stress and DNA damage. Toxicology Letters, 173(2), 80–87. https://doi.org/10.1016/j.toxlet.2007.06.008

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