Purification, characterisation and crystallisation of photosystem II from Thermosynechococcus elongatus cultivated in a new type of photobioreactor

110Citations
Citations of this article
78Readers
Mendeley users who have this article in their library.

Abstract

The thermophilic cyanobacterium Thermosynechococcus elongatus was cultivated under controlled growth conditions using a new type of photobioreactor, allowing us to optimise growth conditions and the biomass yield. A fast large-scale purification method for monomeric and dimeric photosystem II (PSII) solubilized from thylakoid membranes of this cyanobacterium was developed using fast protein liquid chromatography (FPLC). The obtained PSII core complexes (PSIIcc) were analysed for their pigment stoichiometry, photochemical and oxygen evolution activities, as well as lipid and detergent composition. Thirty-six chlorophyll a (Chla), 2 pheophytin a (Pheoa), 9± 1 β-carotene (Car), 2.9±0.8 plastoquinone 9 (PQ9) and 3.8±0.5 Mn were found per active centre. For the monomeric and dimeric PSIIcc, 18 and 20 lipid as well as 145 and 220 detergent molecules were found in the detergent shell, respectively. The monomeric and dimeric complexes showed high oxygen evolution activity with 1/4 O 2 released per 37-38 Chla and flash in the best cases. Crystals were obtained from dimeric PSIIcc by a micro-batch method. They diffract synchrotron X-rays to a maximum resolution of 2.9-Å, resulting in complete data sets of 3.2 Å resolution. © 2004 Elsevier B.V. All rights reserved.

Cite

CITATION STYLE

APA

Kern, J., Loll, B., Lüneberg, C., DiFiore, D., Biesiadka, J., Irrgang, K. D., & Zouni, A. (2005). Purification, characterisation and crystallisation of photosystem II from Thermosynechococcus elongatus cultivated in a new type of photobioreactor. Biochimica et Biophysica Acta - Bioenergetics, 1706(1–2), 147–157. https://doi.org/10.1016/j.bbabio.2004.10.007

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free