Lipase production bacterial isolate was isolated from soil of service station and identified as Bacillus methylotrophicus PS3 by 16SrRNA with accession number |LN999829.1|. Lipase enzyme was purified by sequential methods of ammonium sulfate precipitation and Sephadex G-100 gel column chromatography. The molecular weight of purified enzyme was 31.40 kDa on SDS-PAGE. This purification procedure resulted in 2.90-fold purification of lipase with a 24.10% final yield. The purified lipase presented maximal hydrolytic activity at a temperature of 55 ° C, and pH of 7.0. Lipase activity was stimulated by Triton X-100 and SDS with Mg 2+ and Ca 2+ metals employ a positive effect and outlast its stable in organic solvent i.e. methanol and ethanol.
Sharma, P., Sharma, N., Pathania, S., & Handa, S. (2017). Purification and characterization of lipase by Bacillus methylotrophicus PS3 under submerged fermentation and its application in detergent industry. Journal of Genetic Engineering and Biotechnology, 15(2), 369–377. https://doi.org/10.1016/j.jgeb.2017.06.007