Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells. © 2001 Pubblished by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
Van Hijum, S. A. F. T., Bonting, K., Van Der Maarel, M. J. E. C., & Dijkhuizen, L. (2001). Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced. FEMS Microbiology Letters, 205(2), 323–328. https://doi.org/10.1016/S0378-1097(01)00490-6