Response surface methodology: Optimisation of antifungal bioemulsifier from novel bacillus thuringiensis

2Citations
Citations of this article
15Readers
Mendeley users who have this article in their library.

Abstract

An antifungal bioemulsifier compound was produced from a novel strain of Bacillus thuringiensis pak2310. To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production. The E24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification. A quadratic equation was employed to express the response variable in terms of the independent variables. Statistical tools like student’s t -test, F -test, and ANOVA were employed to identify the important factors and to test the adequacy of the model. Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.

Cite

CITATION STYLE

APA

Rajendran, D., Venkatachalam, P., & Ramakrishnan, J. (2014). Response surface methodology: Optimisation of antifungal bioemulsifier from novel bacillus thuringiensis. Scientific World Journal, 2014. https://doi.org/10.1155/2014/423289

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free