The neem tree has long been recognized for its unique properties, both against insects and in improving human health. Every part of the tree has been used as a traditional medicine for household remedy against various human ailments, from antiquity. Although the occurrence of various phytochemicals in neem has been studied, we have identified the presence of a novel tripeptide in the young leaves of neem using a simple and inexpensive paper chromatographic method, detected by Cu(II)-ninhydrin reagent. The peptide nature of the isolated compound is confirmed by spectral studies. The sequence of the peptide is determined using de novo sequencing by tandem MS after purification. © 2013 M. Rajeswari Prabha and B. Ramachandramurty.
Rajeswari Prabha, M., & Ramachandramurty, B. (2013). Sequence determination of a novel tripeptide isolated from the young leaves of azadirachta indica a. juss. International Journal of Peptides, 2013. https://doi.org/10.1155/2013/629549