The in vitro trypanocidal activity of a 1 : 4 mixture of lupenone and caryophyllene oxide confirmed a synergistic effect of the terpenoids against epimastigotes forms of T. cruzi ( IC50=10.4 μ g/mL, FIC = 0.46). In addition, testing of the terpenoid mixture for its capacity to reduce the number of amastigote nests in cardiac tissue and skeletal muscle of infected mice showed a reduction of more than 80% at a dose level of 20.8 mg·kg −1 ·day −1 .
Polanco-Hernández, G., Escalante-Erosa, F., García-Sosa, K., Rosado, M. E., Guzmán-Marín, E., Acosta-Viana, K. Y., … Peña-Rodríguez, L. M. (2013). Synergistic effect of lupenone and caryophyllene oxide against Trypanosoma cruzi. Evidence-Based Complementary and Alternative Medicine, 2013. https://doi.org/10.1155/2013/435398