A new IS-anionic stationary phase was synthesized to make on-line extraction of the ochratoxin A and B from samples of beer for HPLC. The propyltriethylammonium chloride stationary phase was characterized affecting it's elementary determination and RI specter, respectively. Evaluation of the IS-anionic column for the extraction and quantification of OTA and OTB in beer has shown that the column is suitable for efficient extraction (recovery >76.5%) and precise analysis. The detection limits for OTA and OTB were 0.03 and 0.07 mugL(-1), respectively. The range of detector linearity was 0.03 at 20 mugL(-1).
Menezes, M. L., Simionato, E. M. R. S., & Felix, G. (2008). Synthesis of the stationary phase IS-anionic (internal surface-anionic) for extraction of ochratoxin A and B from samples of beers. Journal of Liquid Chromatography and Related Technologies, 31(17), 2603–2611. https://doi.org/10.1080/10826070802352827