A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells

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Abstract

Epstein-Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8,000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B cell proteome and EBV virome. Our approach revealed EBV-induced remodeling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi-sarcoma-associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.

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APA

Ersing, I., Nobre, L., Wang, L. W., Soday, L., Ma, Y., Paulo, J. A., … Gewurz, B. E. (2017). A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells. Cell Reports, 19(7), 1479–1493. https://doi.org/10.1016/j.celrep.2017.04.062

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