Transport properties of mitochondrial porin were investigated on the basis of changes in the activity of hexokinase utilizing external ATP. Production of glucose: 6-phosphate is inhibited by polyanion both in intact brain mitochondria and in contact point vesicles. Hexokinase activity is restored by solubilization of the enzyme by high ionic strength or 0.5-1% Triton X-100. In very low concentrations (0.001-0.005%) Triton does not mobilize hexokinase from its binding sites but it is able to release polyanion-inhibition completely. This finding provides an explanation for the discrepancy observed in the transport properties of porin when studied 'in situ' or in artificial lipid membranes. © 1995.
CITATION STYLE
Bathori, G., Fonyò, A., & Ligeti, E. (1995). Trace amounts of Triton X-100 modify the inhibitor sensitivity of the mitochondrial porin. BBA - Biomembranes, 1234(2), 249–254. https://doi.org/10.1016/0005-2736(94)00295-Z
Mendeley helps you to discover research relevant for your work.