TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport

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Abstract

TRAPP is a conserved protein complex required early in the secretory pathway. Here, we report two forms of TRAPP, TRAPP I and TRAPP II, that mediate different transport events. Using chemically pure TRAPP I and COPII vesicles, we have reconstituted vesicle targeting in vitro. The binding of COPII vesicles to TRAPP I is specific, blocked by GTPγS, and, surprisingly, does not require other tethering factors. Our findings imply that TRAPP I is the receptor on the Golgi for COPII vesicles. Once the vesicle binds to TRAPP I, the small GTP binding protein Ypt1p is activated and other tethering factors are recruited.

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Sacher, M., Barrowman, J., Wang, W., Horecka, J., Zhang, Y., Pypaert, M., & Ferro-Novick, S. (2001). TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport. Molecular Cell, 7(2), 433–442. https://doi.org/10.1016/S1097-2765(01)00190-3

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