Trypanosome mitochondrial 3′ terminal uridylyl transferase (TUTase): The key enzyme in U-insertion/deletion RNA editing

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Abstract

A 3′ terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3′ U-addition in the presence of Mg2+. TUTase is present in vivo in at least two stable configurations: one contains a ∼500 kDa TUTase oligomer and the other a ∼700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.

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Aphasizhev, R., Sbicego, S., Peris, M., Jang, S. H., Aphasizheva, I., Simpson, A. M., … Simpson, L. (2002). Trypanosome mitochondrial 3′ terminal uridylyl transferase (TUTase): The key enzyme in U-insertion/deletion RNA editing. Cell, 108(5), 637–648. https://doi.org/10.1016/S0092-8674(02)00647-5

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