Tubular Liposomes with Variable Permeability for Reconstitution of FtsZ Rings

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Abstract

We have developed a system for producing tubular multilamellar liposomes that incorporate the protein FtsZ on the inside. We start with a mixture of spherical multilamellar liposomes with FtsZ initially on the outside. Shearing forces generated by applying a coverslip most likely distort some of the spherical liposomes into a tubular shape, and causes some to leak and incorporate FtsZ inside. We describe protocols for liposome preparation, and for preparing membrane-targeted FtsZ that can assemble contractile Z rings inside the tubular liposomes. We also describe the characterization of the multilamellar liposomes in terms of the permeability or leakiness for a small fluorescent dye and larger protein molecules. These liposomes may be useful for reconstitution of other biological systems. © 2009 Elsevier Inc. All rights reserved.

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Osawa, M., & Erickson, H. P. (2009). Tubular Liposomes with Variable Permeability for Reconstitution of FtsZ Rings. Methods in Enzymology. https://doi.org/10.1016/S0076-6879(09)64001-5

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