Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.
CITATION STYLE
Tacke, S., Krzyzanek, V., Nüsse, H., Wepf, R. A., Klingauf, J., & Reichelt, R. (2016). A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics. Biophysical Journal, 110(4), 758–765. https://doi.org/10.1016/j.bpj.2016.01.024
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