Vibrational analysis of the structure of gramicidin A. II. Vibrational spectra

51Citations
Citations of this article
12Readers
Mendeley users who have this article in their library.

Abstract

Raman and infrared spectra have been obtained of gramicidin A (GA) in the crystalline state both in the native form and complexed with CsSCN and KSCN, in solution in dioxane, and incorporated into lipid vesicles. Based on predictions from normal mode calculations of a number of relevant single- and double-stranded beta-helix conformations (Naik and Krimm, 1986), it has been possible to assign the structures of GA that are present under the above conditions. In the crystalline state, native GA has a double-stranded increases decreases beta 5.6 structure, whereas complexes with CsSCN or KSCN adopt a increases decreases beta 7.2 structure. In dioxane solution, the increases decreases beta 5.6 structure predominates. In lipid vesicles, the single-stranded beta 6.3-helix is found, which converts to a double-stranded helix on drying the sample. These results support our previous studies in showing that normal mode analysis can be a powerful technique in obtaining three-dimensional structural information from vibrational spectra. © 1986, The Biophysical Society. All rights reserved.

Cite

CITATION STYLE

APA

Naik, V. M., & Krimm, S. (1986). Vibrational analysis of the structure of gramicidin A. II. Vibrational spectra. Biophysical Journal, 49(6), 1147–1154. https://doi.org/10.1016/S0006-3495(86)83743-2

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free