There are currently very few test objects suitable for high-frequency ultrasound scanners that can be rapidly manufactured, have appropriate acoustic characteristics and are suitably robust. Here we describe techniques for the creation of a wall-less flow phantom using a physically robust konjac and carrageenan-based tissue-mimicking material. Vessel dimensions equivalent to those of mouse and rat arteries were achieved with steady flow, with the vessel at a depth of 1.0mm. We then employed the phantom to briefly investigate velocity errors using pulsed wave Doppler with a commercial preclinical ultrasound system. This phantom will provide a useful tool for testing preclinical ultrasound imaging systems.
Kenwright, D. A., Laverick, N., Anderson, T., Moran, C. M., & Hoskins, P. R. (2015). Wall-less flow phantom for high-frequency ultrasound applications. Ultrasound in Medicine and Biology, 41(3), 890–897. https://doi.org/10.1016/j.ultrasmedbio.2014.09.018