Affinity chromatography of porcine pepsin A using quinolin-8-ol as ligand

  • Novotná L
  • Hrubý M
  • Beneš M
 et al. 
  • 4

    Readers

    Mendeley users who have this article in their library.
  • 3

    Citations

    Citations of this article.

Abstract

Stationary phase containing quinolin-8-ol immobilized on macroporous methacrylate support for the affinity chromatography of porcine pepsin A is described. Optimized chromatographic conditions for separation of porcine pepsin A on this stationary phase were found investigating the influence of pH, concentration, ionic strength and chemical composition of the used mobile phases. The stationary phase shows a good reproducibility of chromatographic analyses (relative standard deviation, ±2%), a high recovery (ca. 93%) and a satisfactory capacity (13 mg pepsin A/1 mL stationary phase) for porcine pepsin A. The obtained findings confirm the applicability of affinity chromatography on the stationary phase with immobilized quinolin-8-ol to the isolation and determination of porcine pepsin A. © 2004 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Affinity chromatography
  • Porcine pepsin A
  • Quinolin-8-ol

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Lenka Novotná

  • Martin Hrubý

  • Milan J. Beneš

  • Zdenka Kučerová

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free