Affinity chromatography of porcine pepsin A using quinolin-8-ol as ligand

  • Novotná L
  • Hrubý M
  • Beneš M
 et al. 
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Stationary phase containing quinolin-8-ol immobilized on macroporous methacrylate support for the affinity chromatography of porcine pepsin A is described. Optimized chromatographic conditions for separation of porcine pepsin A on this stationary phase were found investigating the influence of pH, concentration, ionic strength and chemical composition of the used mobile phases. The stationary phase shows a good reproducibility of chromatographic analyses (relative standard deviation, ±2%), a high recovery (ca. 93%) and a satisfactory capacity (13 mg pepsin A/1 mL stationary phase) for porcine pepsin A. The obtained findings confirm the applicability of affinity chromatography on the stationary phase with immobilized quinolin-8-ol to the isolation and determination of porcine pepsin A. © 2004 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Affinity chromatography
  • Porcine pepsin A
  • Quinolin-8-ol

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  • Lenka Novotná

  • Martin Hrubý

  • Milan J. Beneš

  • Zdenka Kučerová

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