The association of ribulose-1,5-bisphosphate carboxylase with phosphoriboisomerase and phosphoribulokinase

  • Sainis J
  • Harris G
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Abstract

RuBPCase from peas showed Ribose-5-phosphate and Ribulose-5-phosphate dependent CO2fixation when purified on sucrose gradients or by conventional methods. If purification was done in the presence of 20 mM MgCl2and 20-25 mM NaHCO3RuBPCase showed higher Ribose-5-phosphate and Ribulose-5-phosphate dependent CO2fixation rates. Partially purified phosphoriboisomerase, phosphoribulokinase and RuBPCase from spinach could be reassociated in vitro. © 1986 Academic Press, Inc.

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Authors

  • Jayashree K. Sainis

  • Gary C. Harris

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