Characterisation of rubella virus hemagglutinin rosettes

  • Trudel M
  • Marchessault F
  • Payment P
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Purified rubella virus treated with Triton X-100 was subjected to centrifugation in a sucrose density gradient containing nonionic detergent β-D-octylglucoside. The result of this treatment was the formation of hemagglutinating rosettes containing viral glycoproteins VP2(50,000 mol. wt.) and VP3(63,000 mol. wt.). The rosettes have a 26 S sedimentation coefficient and a density of 1.2 g/cm3in sucrose. Electron microscopy revealed 15 nm rosettes with a hollow center. The molecular weight of the rosettes was extrapolated at 850,00. © 1981.

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  • M. Trudel

  • F. Marchessault

  • P. Payment

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