Chimeric drosophila adults produced by transplantation of nuclei into specific regions of fertilized eggs

  • Okada M
  • Kleinman I
  • Schneiderman H
  • 2

    Readers

    Mendeley users who have this article in their library.
  • 14

    Citations

    Citations of this article.

Abstract

When genetically labeled nuclei from the anterior of donor embryos of Drosophila melanogaster at the nuclear multiplication stage were transplanted to the posterior pole of host embryos, chimeric adults were produced in which labeled nuclei participated in forming specific posterior adult structures including germ cells. The spatial patterns of the chimeras resembled those described in gynandromorphs produced by X-chromosome elimination, except that the donor tissue always occupied less than half of the animal. The donor phenotype was usually localized in one area indicating that the nuclei do not migrate separately but tend to stay together with only a few exceptions. The results strongly support the idea that the nuclei at the stage of nuclear multiplication are totipotent and that they acquire different fates according to the region of the egg periplasm that they occupy. The method can be applied to produce chimeras of autosomal mutants for which mosaics are difficult to obtain by genetic techniques. © 1974 Academic Press, Inc.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • M. Okada

  • I. A. Kleinman

  • H. A. Schneiderman

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free