Compartmentation of inhibitors of proteinases A and B and carboxypeptidase Y in yeast

Abstract

After centrifugal fractionation at 40,000 × g of a metabolic lysate from yeast spheroplasts proteinases A and B, and carboxypeptidase Y were found exclusively in the sediment, whereas inhibitors of these proteinases were present only in the supernatant. Immunoprecipitation with an antiserum prepared against the pure heat-stable proteinase B-inhibitor occured in the supernatant but not in the extract of the particulate fraction. © 1974.