Condensation nucleation light scattering detection for biogenic amines separated by ion-exchange chromatography

  • Sadain S
  • Koropchak J
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Abstract

A sensitive method of analysis for biogenic amines, putrescine, cadaverine, histamine and an amino acid precursor, histidine is described herein using ion-exchange chromatography and condensation nucleation light scattering detection. The method was successfully used for the analysis of biogenic amines in fish samples. The method offers a number of advantages: fast elution of analytes with no need for mobile phase conductivity suppression, no derivatization and no electrochemical activity for the analyte's detection. The 3σ detection limits for these compounds were found to range from 8 to 20 ng/ml. Copyright (C) 1999.

Author-supplied keywords

  • Biogenic amines
  • Condensation nucleation light scattering detection
  • Detection, LC
  • Fish
  • Food analysis

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Authors

  • Salma K. Sadain

  • John A. Koropchak

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