Determination of glycosylated hemoglobin by affinity electrophoresis on agarose gel

  • Aleyassine H
  • 2

    Readers

    Mendeley users who have this article in their library.
  • 2

    Citations

    Citations of this article.

Abstract

We describe a method for electrophoretic determination of glycosylated hemoglobin (GHb) on agarose gel membrane. Clear separation of GHb from non-GHb is achieved after 30 min electrophoresis at 60 V using a sodium citrate buffer solution (34 mmol/1, pH 6.5) containing 0.6 g/1 dextran sulfate. The results obtained by this method correlate well with those by agar gel electrophoresis (r = 0.98) and column chromatography (r = 0.96). However, unlike column Chromatographic methods, GHb values obtained on agarose gel are not affected by fluctuations in ambient temperature (18-28°C), changes in pH (6.2-6.6) and ionic strength of buffer solution (26-40 mmol/1), or variant hemoglobin S and C. Also, electrophoresis on agarose gel membrane eliminates the lengthy preparative steps required for cellulose acetate electrophoresis. We conclude that agarose gel electrophoresis is a simple method for quantitative determination of GHb. © 1984.

Author-supplied keywords

  • Affinity electrophoresis
  • Agarose gel
  • Diabetes mellitus
  • Glycosylated hemoglobin determination

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • H. Aleyassine

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free