Differential effects of aminoglycosides on cistron-specific initiation of protein synthesis

Abstract

The 30S initiation complex formation on f2 RNA is inhibited by kasugamycin, kanamycin and gentamicin; but not by streptomycin. The fMet-tRNA-stimulated binding of f2 RNA to the 30S ribosomal subunit is blocked by kasugamycin; but not by kanamycin and gentamicin. Kasugamycin inhibits initiation of translation of maturation protein cistron more markedly than that of coat protein cistron. Kanamycin and gentamicin block initiation of coat protein synthesis, but do not significantly affect initiation of maturation protein synthesis. Streptomycin inhibits both protein syntheses at the same level. © 1972.