Distribution of two transposon-like elements in rice

  • Wang X
  • Shen G
  • Zheng F
 et al. 
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Abstract

We have previously identified two transposon-like elements, RTL-1 and RTL-2, by comparison of the Wx genes of cultivated Asian rice with that of Oryza L.f. spontanea. In this paper we report the distribution and organization of RTL-1 and RTL-2 in the genomes and Wx genes of the genus Oryza (AA, BB, BBCC, CC, CCDD, EE). Southern blot analyses indicate that RTL-1 and RTL-2 are interspersed in all AA genomes of wild rice examined. It is noteworthy that hybridization patterns and copy numbers of RTL-2 are different in some rice species. PCR amplifications using primers in exon regions flanking intron 10 or intron 13 indicate that neither RTL-1 nor RTL-2 is present in the corresponding site of the Wx genes of BB, BBCC, CC, CCDD, EE genome-type of rice. RTL-1 is only present in the Wx gene of O. nivara, O. rufipogon (Guangdong, crawl), while RTL-2 present in the corresponding site of Wx genes of all AA genome-type. Surprisingly we found and proved by sequence analysis that in O. rufipogon from Sri Lanka there are two bands (161 bp and 300 bp) corresponding to the excision/insertion of RTL-1. © 1993.

Author-supplied keywords

  • PCR amplification
  • Repetitive element
  • Southern blotting analysis
  • Transposon
  • Wx gene

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Authors

  • Xiao quan Wang

  • Ge zhi Shen

  • Fei qing Zheng

  • Zong yang Wang

  • Jing liu Zhang

  • Meng min Hong

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