Double evaluations of chemicals using a cocktail of fused recombinant receptors

  • Matsui K
  • Ishibashi T
  • Oka M
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Abstract

Some chemicals have multipotential as endocrine-disrupting chemicals (EDCs). For example, some chemicals act as both estrogens and antiandrogens. Numbers of such chemicals should be evaluated from many aspects; however, labor and expenses are generally limited. We have developed two expression systems for the wild type of human estrogen receptor α and the wild type of human androgen receptor fused with a maltose binding protein. They are soluble and have binding activities. They showed dose-responses to natural hormones and well-known potential EDCs. After we established each assay condition for a competitive binding assay using each receptor, we found that two assay systems can be carried out simultaneously under limited and harmonized conditions. Under harmonized conditions using a cocktail of two types of receptors, we could estimate natural hormones and potential EDCs. Interference between two assay systems was not observed under these conditions. We believe that some competitive binding assays can be carried out using a cocktail of receptors at the same time if interference among different assay systems can be avoided by choosing ideal conditions. © 2002 Elsevier Science (USA). All rights reserved.

Author-supplied keywords

  • Drug discovery
  • Endocrine disruptor
  • Nuclear receptor
  • Protein engineering
  • Screening

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Authors

  • Kazuhiro Matsui

  • Takuya Ishibashi

  • Masanori Oka

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