Epithelial V-like antigen regulates permeability of the blood-CSF barrier

  • Chatterjee G
  • Carrithers L
  • Carrithers M
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Epithelial V-like antigen (EVA), a CD3-binding immunoglobulin-like protein, regulates embryonic thymic development. Here we demonstrate that EVA is expressed in choroid plexus from mature immune competent and lymphocyte-deficient (RAG-/-) mice. Choroid plexus epithelial cells from RAG-/- mice demonstrated reduced junctional integrity and enhanced permeability that was associated with decreased expression of E-cadherin and EVA mRNA as compared to wild-type mice. Following iv infusion of an anti-CD3 antibody (145-2C11) that also binds EVA, expression of E-cadherin and EVA mRNA approached levels seen in wild-type mice. Immuno-fluorescent staining for cadherin also revealed decreased expression in untreated RAG-/- mice that could be increased by 145-2C11 treatment. Expression of mouse EVA in HEK-293 cells followed by challenge with 145-2C11 resulted in increased cytosolic calcium that was not seen in control cells. These results suggest that EVA expressed in choroid plexus cells may regulate the permeability of the blood-CSF barrier. © 2008 Elsevier Inc. All rights reserved.

Author-supplied keywords

  • Blood-CSF barrier
  • Cadherin
  • Choroid plexus
  • Cytosolic calcium
  • Epithelial V-like antigen
  • F-actin
  • Signal transduction

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  • Gouri Chatterjee

  • Lisette M. Carrithers

  • Michael D. Carrithers

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