Epithelial V-like antigen regulates permeability of the blood-CSF barrier

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Abstract

Epithelial V-like antigen (EVA), a CD3-binding immunoglobulin-like protein, regulates embryonic thymic development. Here we demonstrate that EVA is expressed in choroid plexus from mature immune competent and lymphocyte-deficient (RAG-/-) mice. Choroid plexus epithelial cells from RAG-/- mice demonstrated reduced junctional integrity and enhanced permeability that was associated with decreased expression of E-cadherin and EVA mRNA as compared to wild-type mice. Following iv infusion of an anti-CD3 antibody (145-2C11) that also binds EVA, expression of E-cadherin and EVA mRNA approached levels seen in wild-type mice. Immuno-fluorescent staining for cadherin also revealed decreased expression in untreated RAG-/- mice that could be increased by 145-2C11 treatment. Expression of mouse EVA in HEK-293 cells followed by challenge with 145-2C11 resulted in increased cytosolic calcium that was not seen in control cells. These results suggest that EVA expressed in choroid plexus cells may regulate the permeability of the blood-CSF barrier. © 2008 Elsevier Inc. All rights reserved.

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Chatterjee, G., Carrithers, L. M., & Carrithers, M. D. (2008). Epithelial V-like antigen regulates permeability of the blood-CSF barrier. Biochemical and Biophysical Research Communications, 372(3), 412–417. https://doi.org/10.1016/j.bbrc.2008.05.053

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