Epoxy resin coatings for capillary zone electrophoretic separation of basic proteins

27Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Epoxy resins, combining with a cross-linker, were coated on organosilanes modified fused-silica capillaries. After cross-linking, a tough three-dimensional network was attached to capillary surface via Si-O-Si-C bonds. The most effective coating consisted of epoxy resins cross-linked with diaminodiphenylmethane, which was stable over the pH range 2-12 and suppressed protein adsorption effectively. The electroosmotic flow was also greatly reduced and maintained constant over the pH range 6-10. High resolutions and separation efficiencies for basic proteins were obtained in the pH range 3-10 and reproducible separations were achieved from run to run and day to day.

Cite

CITATION STYLE

APA

Liu, Y., Fu, R., & Gu, J. (1996). Epoxy resin coatings for capillary zone electrophoretic separation of basic proteins. Journal of Chromatography A, 723(1), 157–167. https://doi.org/10.1016/0021-9673(95)00825-X

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free