Epoxy resin coatings for capillary zone electrophoretic separation of basic proteins

  • Liu Y
  • Fu R
  • Gu J
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Abstract

Epoxy resins, combining with a cross-linker, were coated on organosilanes modified fused-silica capillaries. After cross-linking, a tough three-dimensional network was attached to capillary surface via Si-O-Si-C bonds. The most effective coating consisted of epoxy resins cross-linked with diaminodiphenylmethane, which was stable over the pH range 2-12 and suppressed protein adsorption effectively. The electroosmotic flow was also greatly reduced and maintained constant over the pH range 6-10. High resolutions and separation efficiencies for basic proteins were obtained in the pH range 3-10 and reproducible separations were achieved from run to run and day to day.

Author-supplied keywords

  • Basic proteins
  • Capillary electrophoresis
  • Coating methods
  • Column coating
  • Proteins

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Authors

  • Yu Liu

  • Ruonong Fu

  • Junling Gu

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