Epoxy resins, combining with a cross-linker, were coated on organosilanes modified fused-silica capillaries. After cross-linking, a tough three-dimensional network was attached to capillary surface via Si-O-Si-C bonds. The most effective coating consisted of epoxy resins cross-linked with diaminodiphenylmethane, which was stable over the pH range 2-12 and suppressed protein adsorption effectively. The electroosmotic flow was also greatly reduced and maintained constant over the pH range 6-10. High resolutions and separation efficiencies for basic proteins were obtained in the pH range 3-10 and reproducible separations were achieved from run to run and day to day.
CITATION STYLE
Liu, Y., Fu, R., & Gu, J. (1996). Epoxy resin coatings for capillary zone electrophoretic separation of basic proteins. Journal of Chromatography A, 723(1), 157–167. https://doi.org/10.1016/0021-9673(95)00825-X
Mendeley helps you to discover research relevant for your work.