An evaluation of the alkaline p-hydroxybenzoic acid hydrazide procedure for the determination of reducing sugars

Abstract

The use of alkaline p-hydroxybenzoic acid hydrazide (HBAH) for the determination of reducing sugars in extracts of plant tissues offers an attractive alternative method to those currently employed. The procedure has good selectivity for reducing sugars, a broad temperature range for maximal colour development, tolerance for ethanol concentrations in the sample up to 40% ( v v), and interferences limited to chloroform and to high protein and calcium concentrations. Further, the range of the method is easily adjusted so that reducing sugar determinations may be performed on a macro- or micro-scale. © 1981.