Evidence for a homogeneous lateral distribution of lipids in a bacterial membrane. A photo cross-linking approach using anthracene as a photoactivable group

  • de Bony J
  • Martin G
  • Welby M
 et al. 
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Abstract

A new photo cross-linking method has been developed for the study of the lateral distribution of lipids in natural membranes, which uses anthracene as a photoactivable group. This method, which rests on the potentiality of anthracene to form covalently bound dimers upon irradiation around 340-380 nm has been applied to the membrane lipids (dimannosyl diacylglycerol, phosphatidylglycerol, phosphatidylinositol) of the bacterium Micrococcus luteus. These glyco- and phospholipids were anthracene labelled by metabolically incorporating the synthetic 9-(2-anthryl)nonanoic acid. The following sequential procedure was used: (i) dimerization of the anthracene-labelled lipids in the membrane by irradiation of the intact cells at 360 nm; (ii) extraction of the lipids and thin-layer chromatography in the first dimension to separate the various lipid dimers from the monomers; (iii) partial dedimerization of the lipid dimers by illumination of the chromatogram at around 250-280 nm; (iv) chromatography in the second dimension to separate the native lipid monomers from the corresponding residual lipid dimers. On account of the occurrence of the 3 hetero dimers phosphatidylglycerol-dimannosyl diacylglycerol, phosphatidylinositol-dimannosyl diacylglycerol and phosphatidylglycerol-phosphatidylinositol after irradiating the cells, it is concluded that in this bacterial membrane, dimannosyl diacylglycerol, phosphatidylglycerol and phosphatidylinositol are homogeneously distributed. © 1984.

Author-supplied keywords

  • Anthracene
  • Glycolipid
  • Lipid lateral distribution
  • Micrococcus luteus
  • Phospholipid
  • Photo cross-linking

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Authors

  • J. de Bony

  • G. Martin

  • M. Welby

  • J. F. Tocanne

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