Evidence that membrane phospholipids and protein are required for binding of diphtheria toxin in Vero cells

6Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Treatment with phospholipase C strongly protected monkey kidney (Vero) cells against diphtheria toxin and reduced the ability of the cells to bind125I-labelled toxin. Treatment with phospholipase D and with trypsin also protected the cells, although to a lesser extent. Phospholipase A2had no protective effect. Phospholipase C also protected fetal hamster kidney cells against the toxin. After removal of the enzymes, as well as after treatment of the cells with 4-acetamide 4′-isothiocyanostilbene 2,2′-disulfonic acid, diphtheria toxin binding capability was restored slowly, apparently by a process requiring protein synthesis, since cycloheximide blocked the restoration. The data indicate that both phospholipids and protein are involved in the binding sites for diphtheria toxin. © 1985.

Cite

CITATION STYLE

APA

Olsnes, S., Carvajal, E., Sundan, A., & Sandvig, K. (1985). Evidence that membrane phospholipids and protein are required for binding of diphtheria toxin in Vero cells. BBA - Molecular Cell Research, 846(3), 334–341. https://doi.org/10.1016/0167-4889(85)90003-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free