Evidence that membrane phospholipids and protein are required for binding of diphtheria toxin in Vero cells

  • Olsnes S
  • Carvajal E
  • Sundan A
 et al. 
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Treatment with phospholipase C strongly protected monkey kidney (Vero) cells against diphtheria toxin and reduced the ability of the cells to bind125I-labelled toxin. Treatment with phospholipase D and with trypsin also protected the cells, although to a lesser extent. Phospholipase A2had no protective effect. Phospholipase C also protected fetal hamster kidney cells against the toxin. After removal of the enzymes, as well as after treatment of the cells with 4-acetamide 4′-isothiocyanostilbene 2,2′-disulfonic acid, diphtheria toxin binding capability was restored slowly, apparently by a process requiring protein synthesis, since cycloheximide blocked the restoration. The data indicate that both phospholipids and protein are involved in the binding sites for diphtheria toxin. © 1985.

Author-supplied keywords

  • Diphtheria toxin
  • Phospholipase
  • Receptor

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  • Sjur Olsnes

  • Encarnacion Carvajal

  • Anders Sundan

  • Kirsten Sandvig

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