On the expression of cytosolic calcium-independent phospholipase A2 (88 kDa) in immature and mature myeloid cells and its role in leukotriene synthesis in human granulocytes

  • Larsson Forsell P
  • Runarsson G
  • Ibrahim M
 et al. 
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Abstract

The human calcium-independent phospholipase A2(iPLA2; 88 kDa) has recently been cloned (Larsson, P.K.A., Claesson, H.-E. and Kennedy, B.P. (1998) J. Biol. Chem. 272, 207-214). Here we demonstrate the expression of the human iPLA2mRNA and its splice variants in blood progenitor cells, immature leukemic cells and mature granulocytes. Chromatographical resolvable iPLA2activity was found in the cytosolic fraction of granulocytes and the activity was inhibited by the iPLA2inhibitor bromoenol lactone. This drug also inhibited leukotriene synthesis in human granulocytes, induced by low concentration of calcium ionophore A23187 (0.10-0.15 μM) or opsonized zymosan. These results suggest that iPLA2is involved in the regulation of the pool of arachidonic acid destined for leukotriene synthesis in human granulocytes. Copyright (C) 1998 Federation of European Biochemical Societies.

Author-supplied keywords

  • Arachidonic acid
  • Bromoenol lactone
  • Leukotriene
  • Phospholipase A2

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Authors

  • Pontus K A Larsson Forsell

  • Gudmundur Runarsson

  • Mirna Ibrahim

  • Magnus Björkholm

  • Hans Erik Claesson

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