Expression efficiency of the human thrombomodulin-encoding gene in various vector and host systems

  • Lin J
  • Wang M
  • Andrews W
 et al. 
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Abstract

Expression systems were developed for evaluating recombinant human thrombomodulin (TM) production in different host cell lines by investigating the performance of five mammalian expression vectors. The expression vectors were constructed so that they contain multiple monocistronic gene cassettes which include a gene encoding a dominant selectable marker, HyR(hygromycin B phosphotransferase), under the regulation of the thymidine kinase promoter, the target gene which encodes a truncated human re-TM under the regulation of various promoters, an amplifiable gene (Dhfr) encoding murine dihydrofolate reductase under the regulation of either the SV40 early or late promoter along with the SV40 enhancer and the SV40 ori. We tested the performance of the five expression vectors in human embryonic kidney cells (HEK293), baby hamster kidney cells (BHK), human melanoma cells (CHL-1) and Dhfr-Chinese hamster ovary cells (CHO/Dhfr-). We found that the efficiency of DNA uptake, transient expression and stable expression of the different expression vectors were all cell-line dependent. However, the myeloproliferative sarcoma virus (MPSV) LTR promoter consistently showed higher expression levels in all cell lines, particularly in HEK293 cells. These results were confirmed by the distribution curves of the level of expression of individual clones. Furthermore, by amplifying Dhfr in transfected CHO/Dhfr-cells with 100 nM methotrexate, we achieved a 20-fold increase in re-TM production using the SV40 late promoter to control murine Dhfr expression. Our data from DNA and mRNA analysis reveal that pMPSV-TM has a high transcription efficiency. Thus, we have developed a versatile expression system that is able to evaluate protein molecules rapidly by transient expression, or by generating stable clones for large-quantity production and is amplifiable for ultimate protein production. © 1994.

Author-supplied keywords

  • Recombinant DNA
  • enhancer amplification
  • expression vectors
  • gene amplification
  • transcription efficiency
  • viral promoters

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Authors

  • Jiing Huey Lin

  • Manping Wang

  • William H. Andrews

  • Robert Wydro

  • John Morser

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